diff --git a/Marlin/src/HAL/shared/eeprom_api.h b/Marlin/src/HAL/shared/eeprom_api.h
index 763271b42d..33d80695db 100644
--- a/Marlin/src/HAL/shared/eeprom_api.h
+++ b/Marlin/src/HAL/shared/eeprom_api.h
@@ -26,8 +26,8 @@
 
 #include "../../libs/crc16.h"
 
-// For testing. Define with -DEEPROM_EXCL_ZONE=919,926 in INI files.
-//#define EEPROM_EXCL_ZONE 919,926  // Test a range
+// For testing. Define with -DEEPROM_EXCL_ZONE=916,926 in INI files.
+//#define EEPROM_EXCL_ZONE 916,926  // Test a range
 //#define EEPROM_EXCL_ZONE 333      // Test a single byte
 
 #ifdef EEPROM_EXCL_ZONE
diff --git a/Marlin/src/pins/stm32f4/pins_CREALITY_V24S1_301F4.h b/Marlin/src/pins/stm32f4/pins_CREALITY_V24S1_301F4.h
index 32f70a3715..fa524464e3 100644
--- a/Marlin/src/pins/stm32f4/pins_CREALITY_V24S1_301F4.h
+++ b/Marlin/src/pins/stm32f4/pins_CREALITY_V24S1_301F4.h
@@ -35,4 +35,6 @@
 #define DISABLE_DEBUG false // DISABLE_(DEBUG|JTAG) is not supported for STM32F4.
 #define ALLOW_STM32F4
 
+#define EEPROM_EXCL_ZONE 916,926  // Ender-3S1 STM32F401 Bootloader EEPROM exclusion zone
+
 #include "../stm32f1/pins_CREALITY_V24S1_301.h"